dsDNA Ab Kit ELISA

N° du produit ABIN996874

Kit ELISA dsDNA Ab Humain, Colorimetric test pour la quantification de Humain dsDNA Ab et a été mentionné dans 1 publication.

Aperçu rapide pour dsDNA Ab Kit ELISA (ABIN996874)

Antigène: dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit dsDNA Ab Kit ELISA

Fonction: dsDNA ELISA is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus eruthematosus (SLE).

Analytical Method: Semi-Quantitative

Specificité: 97.9 %

Sensibilité: 100%

Ingrédients: 1. Microplate: wells coated with Purified dsDNA antigen
2. Serum Diluent Type II
3. Positive Control: Human serum or defibrinated plasma
4. Calibrator: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP Conjugate: Goat anti-human IgG and IgM
7. Wash Buffer Type I 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: 1N H2SO4 solution

Matériel non inclus: 1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a (single wavelength 450 nm or dual 600-650)

Information d'application

Indications d'application: Notable features of Autoimmune Disease ELISA Kits:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results

Volume d'échantillon: 10 μL

Durée du test: 1.5 h

Plaque: Pre-coated

Protocole: dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to the wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen in the wells forming antigen-antibody complexes. Excess antibody is removed by washing and followed by the addition of goat anti-human IgG, M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12-14 months

Références pour dsDNA Ab Kit ELISA (ABIN996874)

Salazar, Copeland, Wood, Schmid, Luebke: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." dans: Journal of immunotoxicology, (2012) (PubMed).

Détail du antigène

Antigène: dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))

Autre désignation: DsDNA Antibody

Classe de substances: Antibody, Antibody

Sujet: Autoimmune diseases are categorized into two general groups: One group is characterized by the production of tissue-specific autoantibodies, the other by autoimmune reactivity with normal cell nuclear and/or cytoplasmic antigens with no tissue type specificity. The latter group includes diseases such as mixed connective tissue disease, scleroderma, rheumatoid arthritis, dermatomyositis, polymyositis, SLE and Sjogren syndrome.