Aflatoxin M1 Kit ELISA

N° du produit ABIN997085

Détail du produit Aflatoxin M1 Kit ELISA

Antigène: Aflatoxin M1

Reactivité: Aspergillus

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Fonction: Aflatoxin M1 quantitative test is based on the principle of the enzyme linked immunosorbent assay.

Analytical Method: Quantitative

Sensibilité: < 10 pg/mL

Information d'application

Plaque: Pre-coated

Préparation des réactifs:

Because the standards are concentrated 10x, they have to be diluted by the enclosed standard/sample diluent 1:10 (e.g. 50 μL standard + 450 μL diluent), before using them in the assay procedure.

Procédure de l'essai:

1. Prepare samples as described above.
   2. Pipet 100 μL diluted (1:10) standards or prepared samples in duplicate into the appropriate wells of the microtiter plate. Immediately add 50 μL aflatoxin M1 antibody into each well.
   3. Cover the microtiter plate with a plastic foil and incubate for 60 minutes at room temperature on a microtiter plate shaker (or 90 minutes without shaker).
   4. Wash the plate three times as follows: Discard the contents of the wells (dump or aspirate). Pipet 300 μL of diluted washing solution into each well. After the third repetition empty the wells again and remove residual liquid by striking the plate against a paper towel. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbencies.
   5. Pipet 100 μL of conjugate (anti-rabbit-IgG-HRP) into each well.
   6. Cover the microtiter plate with a plastic foil and incubate for 60 minutes at room temperature on a microtiter plate shaker (or 90 minutes without shaker).
   7. Wash the plate as outlined in 4.
   8. Pipet 100 μL of substrate solution into each well.
   9. Allow the reaction to develop in the dark (e.g. cupboard or drawer, the chromogen is light-sensitive) for 20 minutes at room temperature.
   10. Stop enzyme reaction by adding 100 μL of stop solution (0.5 M H2SO4) into each well. The blue colour will turn yellow upon addition.
   11. After thorough mixing, measure absorbance at 450 nm (reference wavelength 620 nm), using an ELISA reader. The colour is stable for 30 minutes.

Calcul des résultats:

4. The diluted samples must be further converted by the appropriate dilution factor (50 for the above described extraction). The factor is dependent on the sample preparation procedure employed. TYPICAL STANDARD VALUES The following table contains an example for a typical standard curve. The binding is calculated as percent of the absorption of the 0 pg/mL standard. These values are only an example and should not be used instead of the standard curve which has to be measured in every new test. Aflotoxin B1 (pg/mL) ( % binding of 0 ng/mL) 0 100 10 90 40 85 100 70 400 40 1000 25 PERFORMANCE
   

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C

Détail du antigène

Antigène: Aflatoxin M1

Abstract: Aflatoxin M1 Produits