Lhca3 anticorps
Cet anticorps anti-Lhca3 est un anticorps Lapin Polyclonal détectant Lhca3 dans WB. Adapté pour Arabidopsis thaliana, Orge, Green bean, Oryza sativa, Pisum sativum, Spinach et Zea mays. Ce Primary Antibody a été cité dans 2+ publications.
(2 références)
N° du produit ABIN93513
Aperçu rapide pour Lhca3 anticorps (ABIN93513)
Antigène
Lhca3
(psi Type III Chlorophyll A/B-Binding Protein (Lhca3))
Reactivité
Arabidopsis thaliana, Orge, Green bean, Oryza sativa, Pisum sativum, Spinach, Zea mays
Hôte
Lapin
Clonalité
Polyclonal
Application
Western Blotting (WB)
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Réactivité croisée (Details)
- peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.
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Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
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Purification
- affinity purified
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Immunogène
- BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
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Isotype
- IgG
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Indications d'application
- Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 µL of sterile water
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Buffer
- PBS pH 7.4
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Conseil sur la manipulation
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Stock
- -20 °C
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: "Composition and structure of photosystem I in the moss Physcomitrella patens." dans: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).
: "Differential degradation of photosystem I subunits under iron deficiency in rice." dans: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).
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: "Composition and structure of photosystem I in the moss Physcomitrella patens." dans: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).
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- Lhca3 (psi Type III Chlorophyll A/B-Binding Protein (Lhca3))
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Sujet
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AGI Code: At1g61520
The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073. -
Poids moléculaire
- expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana
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UniProt
- Q9SY97
Antigène
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