DYKDDDDK Positive Control Lysate
PC, WB
Whole Cell Lysate
Overexpression Lysate
E. coli
Reactivité: Tag
N° du produit ABIN1607004
N° du produit (Fournisseur): w00-001-383
Aperçu rapide pour DYKDDDDK Positive Control Lysate (ABIN1607004)
Antigène
DYKDDDDK Tag
Espèce de la protéine
Tag
Application
Positive Control (PC), Western Blotting (WB)
Lysed Cells
E. coli
Lysate Type
Overexpression Lysate
Lysate Fraction
Whole Cell Lysate
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Espèce du lysat
- Escherichia coli (E. coli)
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N° du produit (Fournisseur)
- w00-001-383
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Fournisseur
- Rockland
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niveau d'endotoxine
- Low Endotoxin : No
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Indications d'application
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
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Stock
- -80 °C
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Stockage commentaire
- Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
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Date de péremption
- Expiration date is three (3) months from date of opening.
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- DYKDDDDK Tag
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Classe de substances
- Tag
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Sujet
- This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
Antigène
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