Hippocampus (Alzheimer's Disease) Lysate
Aperçu rapide pour Hippocampus (Alzheimer's Disease) Lysate (ABIN2456590)
Application
Lysate Type
Lysate Fraction
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Espèce du lysat
- Human
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Attributs du produit
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Human hippocamps tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The human hippocamps tissue total protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the hippocamps tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The hippocamps tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Source: Human Hippocampus tissue
Diagnosis: Diseased
Tissue or Cell type: Brain: Hippocampus
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Indications d'application
- Hippocampus lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2 mg/mL
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Buffer
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
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Conseil sur la manipulation
- Repeated freezing and thawing is not recommended.
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Stock
- 4 °C/-80 °C
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Stockage commentaire
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
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Date de péremption
- 6 months
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