Lymphoma, Hodgkins Disease Membrane Tumor Lysate
Aperçu rapide pour Lymphoma, Hodgkins Disease Membrane Tumor Lysate (ABIN2456746)
Application
Lysate Type
Lysate Fraction
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Espèce du lysat
- Human
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Attributs du produit
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Human lymphoma, Hodgkins Disease tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human lymphoma, Hodgkins Disease tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The membrane protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated lymphoma, Hodgkins Disease tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated lymphoma, Hodgkins Disease tissue membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal.
Source: Human lymphoma, Hodgkins Disease tissue
Diagnosis: Tumor
Tissue or Cell type: lymphoma, Hodgkins Disease
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Indications d'application
- lymphoma, Hodgkins Disease membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2 mg/mL
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Buffer
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
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Conseil sur la manipulation
- Repeated freezing and thawing is not recommended.
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Stock
- 4 °C/-80 °C
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Stockage commentaire
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
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Date de péremption
- 6 months
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