PC-12 nuclear extract (Hypoxia)
Aperçu rapide pour PC-12 nuclear extract (Hypoxia) (ABIN2669787)
Lysed Cells
Lysate Type
Lysate Fraction
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Espèce du lysat
- Rat
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Attributs du produit
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Origin: rat pheochromocytoma (kidney tumor)
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Ingrédients
- 200 μg of PC-12 (Hypoxia) nuclear extract at 5.0 μg/μl
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Restrictions
- For Research Use only
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Concentration
- 5.0 μg/μL
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Buffer
- Nuclear extracts are supplied in dilution buffer (20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT). Before harvesting the cells were deprived of oxygen for 4 hours.
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Agent conservateur
- Dithiothreitol (DTT)
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Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Stock
- -80 °C
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Stockage commentaire
- To ensure stability, extracts should be stored at -80°C.
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