HeLa nuclear extract (4 hr serum response)
Aperçu rapide pour HeLa nuclear extract (4 hr serum response) (ABIN2669789)
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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Espèce du lysat
- Human Cells
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Attributs du produit
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Origin: Human Cervix Adenocarcinoma
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Ingrédients
- 2 x 100 μg of HeLa nuclear extract (4 hr serum response) at 2.5 μg/μl
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MEIS1 293T Cell Transient Overexpression Lysate(Denatured)
MEIS1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
SLC26A2 293T Cell Transient Overexpression Lysate(Denatured)SLC26A2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
PTGS2 293T Cell Transient Overexpression Lysate(Denatured)PTGS2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
PTGER2 293T Cell Transient Overexpression Lysate(Denatured)PTGER2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
PTGS1 293T Cell Transient Overexpression Lysate(Denatured)PTGS1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
LSAMP 293T Cell Transient Overexpression Lysate(Denatured)LSAMP WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
MICB 293T Cell Transient Overexpression Lysate(Denatured)MICB WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
HRG 293T Cell Transient Overexpression Lysate(Denatured)HRG WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
HOXB5 293T Cell Transient Overexpression Lysate(Denatured)HOXB5 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
MGMT 293T Cell Transient Overexpression Lysate(Denatured)MGMT WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain
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Restrictions
- For Research Use only
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Concentration
- 2.5 μg/μL
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Buffer
- Nuclear extracts are supplied in dilution buffer (20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured for 24 hours in low serum (0.5 %) conditions and then serum-stimulated (10 %) for 4 hours prior to harvesting.
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Agent conservateur
- Dithiothreitol (DTT)
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Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Stock
- -80 °C
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Stockage commentaire
- To ensure stability, extracts should be stored at -80°C.
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