Protein Molecular Weight Marker

Détails pour le produit réf. ABIN925595, Fournisseur: Connectez-vous pour afficher
Gamme de detection
14-120 kDa
Seuil minimal de détection
14 kDa
Application
SDS-PAGE (SDS), Western Blotting (WB)
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Fonction This protein molecular weight marker is a cocktail containing seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits.
Méthode de détection Colorimetric
Attributs du produit The protein mixture has been formulated to yield well-defined bands after SDS-polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques. The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference. The product is supplied in 1X loading buffer and is ready-to-use.
Stérilité Sterile filtered
Indications d'application This product is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats. These are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). 62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
Commentaires

in 1x Loading Buffer

Restrictions For Research Use only
Format Liquid
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Images (Fournisseur)
 image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker
 image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker (Image 2)
SDS-PAGE (SDS) image for Protein Molecular Weight Marker (ABIN925595) SDS-Page of Protein Molecular Weight Marker. Lane 1: Protein Molecular Weight Marker ...
SDS-PAGE (SDS) image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker (Image 4)