Nlrp1a Protein (AA 1-1182) (Strep Tag)
Crystallography grade
NLRP1A
Origine: Souris
Hôte: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
1 image
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- Antigène
- Nlrp1a (NLRP1A) (NLR Family, Pyrin Domain Containing 1A (NLRP1A))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1182
- Origine
- Souris
- Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette Nlrp1a protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MEESQSKQES STKVAQHEGQ EDVDPTFKTK KLMEVELMKH RVQLERNLKL RTFPGARTKQ VKEALYPLLT WSSKSKNLFQ NFTKLLLFKK LCQRGSENLV RESWYPCVPE EEAHMIDIQD LFGPNLGTQK KPQLVIIEGA AGIGKSTLAR LVKRAWKEGK LYRNDFHHVF FFSCRELAQY EQLSLAELIV QGQEVPTAPI RQILSHPEKL LFILDGIDEP AWVLADQNPE LCLHWSQTQP VHTLLGSLLG KSILPGASFL LTTRTTALQK FIPSLEQPCQ VEVLGFTLFE RKNYFYKYFG KKKGGVTTFT LVKSNSALLT LCEVPWVCWL VCTCLKKQME QGGELSLTSQ TTTALCLKYL SLTIPGQHMR TQLRDLCSLA AEGVCQRRTL FSESDLCKQG LDEHAIASFL KIGVLQKQAS SLSYSFAHLC LQEFFAAMSY ILDDSEERHA DMKNDRIVET LVERYGRQNL FEAPTVRFLF GLLSKEELKK IEKLFSCSLH GKTKLKLLWH ILGKSQPHQP PCLGLLHCLY ENQDMELLTH VMHDLQGTIV PGPDDLAHTV LQTNVKHLVI QTDMDLMVVT FCIKFCCHVR SLQLNRKVQQ GHKFTAPGMV LYRWTPITDA SWKIFFSNLK LARNLEELDL SGNPLSYYAV HSLCTTLRKR GCQLKTLWLV ECGLTSTYCS LLASVLSARS SLTELDLQLN DLGDGGVKML CEGLRNPACN LSILWLDQAS LSDQVIAELR TLEAKNPKLL ISSTWKPHVM VPTMNMDKEE VGDSQALLKQ QRQQSGDKHM EPLGTEDEFW GPTGPVTTEV VDRERNLYRV QLPMAGSYHC PSTGLHFVVT RAVTIEIEFC AWSQYLDKTP LQQSHMVVGP LFDIKAEQGA VTAVYLPHFV ALQEGIVDSS LFHVAHFQEH GMVLETPARV EQHYAVLENP SFSPMGILLR MIPAVGHFIP ITSTTLIYYH LYLEDVTFHL YLVPNDCSIR KAIDDEEMKF QFVRINKPPP VDALYLGSRY IVSSSKLVEI IPKELELCYR SPGESQLFSE IDIGHMDSEI KLQIKDKRHM NLKWEALLKP GDLRPALPKI ATAPKDAPSL LHFMDQHREQ LVARVTSVDP LLDKLHGLVL SEDSYEVVRS ETTNQDKMRK LFSLSRSWSW DCKDQFYQAL KETHPHLVMD ILEKLGGVSV KS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Nlrp1a (NLRP1A) (NLR Family, Pyrin Domain Containing 1A (NLRP1A))
- Autre désignation
- Nlrp1a
- Synonymes
- CARD7 Protein, DEFCAP Protein, Gm14 Protein, Gm15 Protein, NAC Protein, Nalp1 Protein, Nalp1a Protein, Nlrp1 Protein, PP1044 Protein, NLR family, pyrin domain containing 1A Protein, Nlrp1a Protein
- Sujet
- NACHT, LRR and PYD domains-containing protein 1a (EC 3.4.-.-) (Caspase recruitment domain-containing protein 7) (Death effector filament-forming ced-4-like apoptosis protein) (Nucleotide-binding domain and caspase recruitment domain) [Cleaved into: NACHT, LRR and PYD domains-containing protein 1a, C-terminus (Nlrp1a-CT), NACHT, LRR and PYD domains-containing protein 1a, N-terminus (Nlrp1a-NT)],FUNCTION: Acts as the sensor component of the Nlrp1a inflammasome, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis (PubMed:23219391). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (By similarity). Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, and mediates the formation of the inflammasome polymeric complex (By similarity). In response to pathogen-associated signals, the N-terminal part of Nlrp1a is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (NACHT, LRR and PYD domains-containing protein 1a, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the inflammasome recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis (By similarity). In the absence of GSDMD expression, the Nlrp1a inflammasome is able to recruit and activate CASP8, leading to activation of gasdermin-E (GSDME) (By similarity). Activation of Nlrp1a inflammasome is also required for HMGB1 secretion, the active cytokines and HMGB1 stimulate inflammatory responses (By similarity). When activated in the bone marrow, induces the pyroptosis of hematopoietic stem cells and progenitor cells of both myeloid and lymphoid lineages, hence allowing the removal of damaged cells, and the release of IL1B, which induces granulopoiesis (PubMed:23219391). {ECO:0000250|UniProtKB:Q9C000, ECO:0000269|PubMed:23219391}., FUNCTION: [NACHT, LRR and PYD domains-containing protein 1a]: Constitutes the precursor of the Nlrp1a inflammasome, which mediates autoproteolytic processing within the FIIND domain to generate the N-terminal and C-terminal parts, which are associated non-covalently in absence of pathogens and other damage-associated signals. {ECO:0000250|UniProtKB:Q9C000}., FUNCTION: [NACHT, LRR and PYD domains-containing protein 1a, N-terminus]: Regulatory part that prevents formation of the Nlrp1a inflammasome: in absence of pathogens and other damage-associated signals, interacts with the C-terminal part of Nlrp1a (NACHT, LRR and PYD domains-containing protein 1a, C-terminus), preventing activation of the Nlrp1a inflammasome (By similarity). In response to pathogen-associated signals, this part is ubiquitinated and degraded by the proteasome, releasing the cleaved C-terminal part of the protein, which polymerizes and forms the Nlrp1a inflammasome (By similarity). {ECO:0000250|UniProtKB:Q9C000}., FUNCTION: [NACHT, LRR and PYD domains-containing protein 1a, C-terminus]: Constitutes the active part of the Nlrp1a inflammasome (By similarity). In absence of pathogens and other damage-associated signals, interacts with the N-terminal part of Nlrp1a (NACHT, LRR and PYD domains-containing protein 1a, N-terminus), preventing activation of the Nlrp1a inflammasome (By similarity). In response to pathogen-associated signals, the N-terminal part of Nlrp1a is degraded by the proteasome, releasing this form, which polymerizes to form the Nlrp1a inflammasome complex: the Nlrp1a inflammasome complex then directly recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, leading to gasdermin-D (GSDMD) cleavage and subsequent pyroptosis (By similarity). {ECO:0000250|UniProtKB:Q9C000}.
- Poids moléculaire
- 134.3 kDa
- UniProt
- Q2LKU9
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