Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) anticorps
L’anticorps Chèvre Polyclonal anti- a été validé pour ELISA, FACS, IF et WB. Il convient pour détecter dans des échantillons de Humain.
N° du produit ABIN185294
Aperçu rapide pour Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) anticorps (ABIN185294)
Antigène
Tous les produits Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
Reactivité
Humain
Hôte
Chèvre
Clonalité
Polyclonal
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
Classe de qualité
Verified
-
-
Épitope
- AA 429-442, AA 443-456
-
Fonction
- LXR alpha / LXR beta
-
Séquence
- CRLQDKKLPP LLSEI
-
Specificité
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
-
Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Rat
-
Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
Immunogène
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
-
Isotype
- IgG
-
-
-
Indications d'application
-
Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
-
Commentaires
-
Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 0.5 mg/mL
-
Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Conseil sur la manipulation
- Minimize freezing and thawing.
-
Stock
- -20 °C
-
Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
-
Autre désignation
- NR1H3, NR1H2
-
Sujet
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
-
ID gène
- 10062, 7376
-
NCBI Accession
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
-
Pathways
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Antigène
-
Vous êtes ici:
