(5 références)Aperçu rapide pour Humain TNF ELISPOT Pair (ABIN2691005)
Antigène
Reactivité
Application
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Marque
- BD™ ELISPOT
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Ingrédients
- This product contains sufficient reagent for five 96-well plates, including unlabelled capture antibody (no azide/low endotoxin format), biotinylated detection antibody, and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.
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TNF (Human) Matched Antibody Pair
ELISA Reactivité: Humain ELISA unconjugated Hôte: Souris
TNF (Human) Matched Antibody PairELISA Reactivité: Humain ELISA unconjugated Hôte: Souris
TNF (Human) Matched Antibody PairELISA Reactivité: Humain ELISA unconjugated Hôte: Souris
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Indications d'application
- The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
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Commentaires
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BD™ ELISPOT Human TNF ELISPOT Pair
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Restrictions
- For Research Use only
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Buffer
- Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
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: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).
: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." dans: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).
: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." dans: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).
: "Determination of herpes simplex virus-induced alpha interferon-secreting human blood leucocytes by a filter immuno-plaque assay." dans: Scandinavian journal of immunology, Vol. 27, Issue 2, pp. 165-70, (1988) (PubMed).
: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." dans: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).
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: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Autre désignation
- TNF
Antigène
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