Rat BMP-2 EZ Set™ ELISA Kit (DIY Antibody Pairs)
Aperçu rapide pour Rat BMP-2 EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510762)
Antigène
Épitope
Reactivité
Hôte
Application
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Méthode de détection
- Colorimetric
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Type de méthode
- Sandwich ELISA
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Fonction
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-2 in Rat samples.
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Marque
- EZ-Set™
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Specificité
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Expression system for standard: CHO
Immunogen sequence: Q283-R396 -
Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Attributs du produit
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Tissue Specificity: Expressed in femur, calvaria, trachea, lung and ovary.
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Ingrédients
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Matériel non inclus
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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Mouse BMP-2 EZ Set™ ELISA Kit (DIY Antibody Pairs)
ELISA Reactivité: Souris ELISA unconjugated AA 283-396 Hôte: Lapin
Human BMP-2 EZ Set™ ELISA Kit (DIY Antibody Pairs)ELISA Reactivité: Humain ELISA unconjugated AA 283-396 Hôte: Lapin
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Commentaires
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Restrictions
- For Research Use only
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Date de péremption
- 12 months
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- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
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Autre désignation
- Bmp2
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Sujet
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Protein Function: Induces cartilage and bone formation.
Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Synonyms: Bone morphogenetic protein 2,BMP-2,Bone morphogenetic protein 2A,BMP-2A,Bmp2,Bmp-2,
Full Gene Name: Bone morphogenetic protein 2
Cellular Localisation: Secreted. -
UniProt
- P49001
Antigène
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