Souris G-CSF EZ Set™ ELISA Kit (DIY Antibody Pairs)
Aperçu rapide pour Souris G-CSF EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510767)
Antigène
Épitope
Reactivité
Hôte
Application
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Méthode de détection
- Colorimetric
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Type de méthode
- Sandwich ELISA
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Fonction
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects G-CSF in Mouse samples.
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Marque
- EZ-Set™
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Specificité
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Expression system for standard: E.coli
Immunogen sequence: V31-A208 -
Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Ingrédients
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Matériel non inclus
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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CSF3 (Human) IP-WB Antibody Pair
IP Reactivité: Humain IP unconjugated Hôte: Lapin, Souris
CSF3 (Human) IP-WB Antibody PairIP Reactivité: Humain IP unconjugated Hôte: Lapin, Souris
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Commentaires
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Restrictions
- For Research Use only
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Date de péremption
- 12 months
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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Autre désignation
- Csf3
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Sujet
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Protein Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Background: Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant Mouse G-csf, consisting of 179 amino acids with the molecular mass of 19KDa.
Synonyms: Granulocyte colony-stimulating factor,G-CSF,Csf3,Csfg,
Full Gene Name: Granulocyte colony-stimulating factor
Cellular Localisation: Secreted. -
UniProt
- P09920
Antigène
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