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Humain SP-D EZ Set™ ELISA Kit (DIY Antibody Pairs)

ELISA Reactivité: Humain ELISA unconjugated AA 21-375 Hôte: Lapin
N° du produit ABIN5510794

Aperçu rapide pour Humain SP-D EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510794)

Antigène

SFTPD (Surfactant Protein D (SFTPD))

Épitope

AA 21-375

Reactivité

Humain

Hôte

  • 4
Lapin

Application

ELISA
  • Méthode de détection

    Colorimetric

    Type de méthode

    Sandwich ELISA

    Fonction

    EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects SP-D in Human samples.

    Marque

    EZ-Set™

    Specificité

    Expression system for standard: NSO
    Immunogen sequence: A21-F375

    Réactivité croisée (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Attributs du produit

    Sequence similarities: Belongs to the SFTPD family.

    Tissue Specificity: Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .

    Ingrédients

    Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)

    Matériel non inclus

    1. Microplate reader in standard size.
    2. Automated plate washer.
    3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
    4. Clean tubes and Eppendorf tubes.
    5. Washing buffer (neutral PBS or TBS).
  • Commentaires

    The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
    The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
    The EZ Set™ Accessory Kit provides:
    Antibody diluent buffer
    ABC diluent buffer
    Sample diluent buffer
    TMB
    TMB stop solution
    5x 96 well plates, plate sealers

    Restrictions

    For Research Use only
  • Buffer

    Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

    Date de péremption

    12 months
  • Antigène

    SFTPD (Surfactant Protein D (SFTPD))

    Autre désignation

    SFTPD

    Sujet

    Protein Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .

    Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. It is mapped to 10q22.2-q23.1. The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism.

    Synonyms: Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,SFTPD,COLEC7, PSPD, SFTP4,

    Full Gene Name: Pulmonary surfactant-associated protein D

    Cellular Localisation: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.

    UniProt

    P35247
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