PARP1 (N-term ZF1) Antibody Set - KCC-GM8
WB, IHC
Reactivité: Humain
WB, IHC
unconjugated
N-Term
Hôte: Lapin
Polyclonal
N° du produit ABIN7565996
276,21 €
Frais d'envoi 40,00 €, glace carbonique 20,00 € et TVA exclus
1 set
Destination:
France
Envoi sous 5 à 8 jours ouvrables
Aperçu rapide pour PARP1 (N-term ZF1) Antibody Set - KCC-GM8 (ABIN7565996)
Antigène
PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
Épitope
N-Term
Reactivité
Humain
Hôte
Lapin
Clonalité
Polyclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC)
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Fonction
- PARP1 (N-term ZF1) Antibody Set
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Attributs du produit
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Synonyms: Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL, primary and secondary antibody pair and control protein, Control protein+Primary+Secondary, matched antibody pair
This PARP1 Antibody Set contains: PARP1 (N-term ZF1) protein, Rabbit Anti-PARP1 Antibody, and Goat Anti-RABBIT IgG (HRP) Antibody. PARP1 (N-term ZF1) protein is an N-terminus His-Tag recombinant protein expressed in E.coli corresponding to a fragment of the human PARP1 zinc finger domain. Rabbit Anti-PARP1 (N-term ZF1) was purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined. Goat Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. -
Stérilité
- Sterile filtered
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Immunogène
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Immunogen: Anti-PARP1 (N-term ZF1) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with n-terminus region of human PARP1 zinc finger domain recombinant protein. Anti-Rabbit IgG HRP secondary antibody was produced by repeated immunizations in goat with Rabbit IgG whole molecule.
Immunogen Type: Recombinant Protein
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Indications d'application
- Application Note: Anti-PARP1 (N-term ZF1) antibody with the matched secondary has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract. PARP1 (N-term ZF1) Control Protein analysis by SDS-PAGE resulted in a band at a MW ~13 kDa and estimated to be greater than 95 % pure by Coomassie staining.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide and 0.01 % (w/v) Gentamicin Sulfate -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Control Protein and Primary antibody: the vial contains a relatively low volume of reagent (25 μL). Store vial at -20° C or below prior to opening. To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Secondary antibody: Store vial at -20° C. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
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Autre désignation
- PARP1
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Sujet
- Background: PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to recruit repair proteins) by PARylation. PARP1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. It has been implicated in a new form of cell death termed parthanatos. PARP1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. Anti-PARP1 (N-term ZF1) antibody is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.
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UniProt
- P09874
Antigène
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