When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
PACSIN3
(Protein Kinase C and Casein Kinase Substrate in Neurons 3 (PACSIN3))
Synonymes
Pacsin1 Peptide, wu:fa11d03 Peptide, wu:fj67e09 Peptide, wu:fq16a06 Peptide, zgc:56324 Peptide, MGC82145 Peptide, PACSIN3 Peptide, sdpiii Peptide, SDPIII Peptide, 4921507A02Rik Peptide, 6330413E15Rik Peptide, AW413130 Peptide, SdpIII Peptide, protein kinase C and casein kinase substrate in neurons 3 Peptide, protein kinase C and casein kinase substrate in neurons 3 L homeolog Peptide, pacsin3 Peptide, PACSIN3 Peptide, pacsin3.L Peptide, Pacsin3 Peptide