When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
ABCF3
(ATP-Binding Cassette, Sub-Family F (GCN20), Member 3 (ABCF3))
Synonymes
ABCF3 Peptide, DKFZp459M1517 Peptide, EST201864 Peptide, AI326318 Peptide, AU016058 Peptide, BB119416 Peptide, ATP binding cassette subfamily F member 3 Peptide, ATP-binding cassette sub-family F member 3 Peptide, ATP-binding cassette, sub-family F (GCN20), member 3 Peptide, ABCF3 Peptide, CpipJ_CPIJ014150 Peptide, PTRG_01254 Peptide, PTRG_00878 Peptide, VDBG_00967 Peptide, MGYG_07201 Peptide, Abcf3 Peptide