When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
VPS25
(Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))
Synonymes
VPS25 Peptide, CG14750 Peptide, Dmel\\CG14750 Peptide, ESCRT-I Peptide, ESCRT-II Peptide, dVps25 Peptide, l(2)44Db Peptide, l(2)k08904 Peptide, vps25 Peptide, GB16268 Peptide, derp9 Peptide, eap20 Peptide, fap20 Peptide, DDBDRAFT_0189483 Peptide, DDBDRAFT_0234026 Peptide, DDB_0189483 Peptide, DDB_0234026 Peptide, 1110020N13Rik Peptide, AW107467 Peptide, D11Wsu68e Peptide, im:6898134 Peptide, zgc:162560 Peptide, DERP9 Peptide, EAP20 Peptide, FAP20 Peptide, vacuolar protein sorting 25 homolog Peptide, vacuolar protein sorting 25 homolog (S. cerevisiae) pseudogene Peptide, vacuolar protein sorting-associated protein 25 Peptide, WNK lysine deficient protein kinase 4 Peptide, Vacuolar protein sorting 25 Peptide, vacuolar protein-sorting-associated protein 25 Peptide, vacuolar protein sorting 25 homolog L homeolog Peptide, vacuolar protein sorting 25 Peptide, vacuolar protein sorting 25 homolog (S. cerevisiae) Peptide, E2F/DP family winged-helix DNA-binding domain-containing protein Peptide, VPS25 Peptide, LOC459431 Peptide, CpipJ_CPIJ010292 Peptide, WNK4 Peptide, Vps25 Peptide, LOC412381 Peptide, vps25.L Peptide, vps25 Peptide