(5 références)-
- Antigène Tous les produits DYKDDDDK Tag
- DYKDDDDK Tag
- Pureté
- > 95 %
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DYKDDDDK Tag peptide (DYKDDDDK Tag)
Reactivité: Tag CP, IP, WB
DYKDDDDK Tag peptideReactivité: Tag CP, IP, WB
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- Restrictions
- For Research Use only
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- Stock
- 4 °C
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: "USP7 controls Chk1 protein stability by direct deubiquitination." dans: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 24, pp. 3921-6, (2015) (PubMed).
: "Assembly and architecture of the EBV B cell entry triggering complex." dans: PLoS pathogens, Vol. 10, Issue 8, pp. e1004309, (2014) (PubMed).
: "An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 8, pp. 3173-8, (2013) (PubMed).
: "A lysine-rich region within fungal BAG domain-containing proteins mediates a novel association with ribosomes." dans: Eukaryotic cell, Vol. 11, Issue 8, pp. 1003-11, (2012) (PubMed).
: "In vivo and in vitro analysis of the hedamycin polyketide synthase." dans: Chemistry & biology, Vol. 16, Issue 11, pp. 1197-207, (2009) (PubMed).
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: "USP7 controls Chk1 protein stability by direct deubiquitination." dans: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 24, pp. 3921-6, (2015) (PubMed).
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- Antigène
- DYKDDDDK Tag
- Classe de substances
- Tag
- Sujet
- A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
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