Aperçu rapide pour Lysyl Oxidase (LOX) (Internal Region) Peptide (ABIN5539397)
Antigène
LOX
(Lysyl Oxidase (LOX))
Origine
Humain
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
Protein Region
Internal Region
Séquence
STAYRADVRD YDHR
Homologie
Human, Pig
Indications d'application
When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 30-33 kDa band observed in Human Heart and Human Placenta lysates (calculated MW of 46.9 kDa according to NP_002308.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kuivaniemi et al, J Biol Ch
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.