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Zinc finger protein ubi-d4 (DPF2) (AA 89-103), (Internal Region) Peptide

DPF2 Reactivité: Humain Hôte: Synthetic BP, Imm
N° du produit ABIN5539411

Aperçu rapide pour Zinc finger protein ubi-d4 (DPF2) (AA 89-103), (Internal Region) Peptide (ABIN5539411)

Antigène

DPF2 (Zinc finger protein ubi-d4 (DPF2))

Origine

Humain

Source

  • 6
Synthetic

Application

Blocking Peptide (BP), Immunogen (Imm)
  • Protein Region

    AA 89-103, Internal Region

    Séquence

    EDPRLSFPSI KPDTD

    Homologie

    Human, Mouse, Rat, Dog, Pig, Cow
  • Indications d'application

    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 50 kDa and 48 kDa bands observed in lysates of cell lines Jurkat and K562 respectively (calculated MW of 44.2 kDa according to NP_006259.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.

    Buffer

    100µg of Lyophilised peptide, formulation De-ionized (DI) water.

    Stock

    -20 °C

    Stockage commentaire

    Shipped at ambient temperature, store at -20°C.
  • Antigène

    DPF2 (Zinc finger protein ubi-d4 (DPF2))

    ID gène

    5977, 19708, 361711

    NCBI Accession

    NP_006259
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