Carbonic Anhydrase 12 (CA12) (AA 188-199), (Internal Region) peptide (Biotin)
CA12
Reactivité: Humain
Hôte: Synthetic
BP, Imm
N° du produit ABIN5539421
Aperçu rapide pour Carbonic Anhydrase 12 (CA12) (AA 188-199), (Internal Region) peptide (Biotin) (ABIN5539421)
Antigène
CA12
(Carbonic Anhydrase 12 (CA12))
Origine
Humain
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- AA 188-199, Internal Region
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Purification/Conjugué
- This CA12 peptide is labeled with Biotin.
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Séquence
- CSHLQHVKYK GQE.
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Homologie
- Human
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Indications d'application
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 45 kDa band observed in Human kidney lysates (calculated MW of 39.5 kDa according to NP_001209.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Stock
- -20 °C
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Stockage commentaire
- Shipped at ambient temperature, store at -20°C.
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- CA12 (Carbonic Anhydrase 12 (CA12))
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ID gène
- 771
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NCBI Accession
- NP_001209, NP_996808, NP_001280571
Antigène
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