N° du produit ABIN7582292
Aperçu rapide pour Tertiapin Q Peptide (ABIN7582292)
Antigène
Tertiapin Q
Origine
Bee
Source
Synthetic
Application
Blocking Peptide (BP)
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Type de proteíne
- Synthetic
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Fonction
- A Potent Blocker of Inward Rectifier (Kir) K+ Channels
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Séquence
- ALCNCNRIII PHQCWKKCGK K-NH2
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Specificité
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Toxin target: Kir1.1, Kir3.2 K+ channels,
Disulfide bonds between: Cys3-Cys14, Cys5-Cys18 Lys21 - C-terminal amidation ATTO Fluor-488 -
Attributs du produit
- A Potent Blocker of Inward Rectifier (Kir) K+ Channels
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Pureté
- ≥98 % (HPLC)
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Tertiapin Q peptide (Atto 633)
Reactivité: Bee Hôte: Synthetic BP, FACS, FM, IF, LCI
Tertiapin Q peptide (Atto 488)Reactivité: Bee Hôte: Synthetic BP, FACS, FM, IF, LCI
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Indications d'application
- Antigen Preadsorption Control: 2 - 200 nM
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Commentaires
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Electrophysiology
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- Tertiapin Q
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Sujet
- TPN-Q,Tertiapin, the native toxin, was originally isolated from European honey bee Apis mellifera venoM Native and synthetic Tertiapin blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 (Kir1.1, IC50 = 2 nM) and GIRK (Kir3 family, IC50 for the Kir3.1/3.4 heteromer was 8.6 nM) but with no effect on the Kir2 family member1. In accordance, it was shown to inhibit acetylcholine induced K+ currents in mammalian cardiomyocytes2,3.Tertiapin-Q is a derivative of Tertiapin in which Met13 is substituted by a Gln residue. However, unlike native Tertiapin, Tertiapin-Q is non-oxidizable and therefore is more stable4.Tertiapin-Q inhibits the above-mentioned channels with similar affinities and also inhibits Ca2-activated large conductance BK-type K+ channels in a concentration and voltage-dependent manner5.
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Poids moléculaire
- 2452 Da
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UniProt
- P56587
Antigène
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