Purified recombinant E.coli DsbE protein Expression System: E.coli Molecular weight on SDS-PAGE will appear higher.
Pureté
> 95 % pure
Indications d'application
DsbE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM EDTA, and 10 % glycerol.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
RT/-20 °C
Stockage commentaire
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Antigène
DsbE
Sujet
DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography. Alternative Names: yejQ protein, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187 protein, Periplasmic thioredoxin of cytochrome c type biogenesis protein, CcmG protein, JW2183.