Purified recombinant S.aureus Protein A protein Expression System: E.coli Molecular weight on SDS-PAGE will appear higher.
Pureté
> 90 % pure
niveau d'endotoxine
< 1.0 EU per µg of protein (determined by LAL method)
Indications d'application
Protein A protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
RT/-20 °C
Stockage commentaire
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Antigène
Protein A
Synonymes
alpha protein Peptide, repB Peptide
Sujet
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography. Alternative Names: SPA protein, Immunoglobulin G binding protein A protein, , Immunoglobulin G binding protein A Staphylococcal protein A protein