FK506 Binding Protein 2, 13kDa (FKBP2) (AA 22-142) Peptide
FKBP2
Reactivité: Humain
Hôte: Escherichia coli (E. coli)
SDS
Dithiothreitol (DTT)
1 image
N° du produit ABIN935096
Aperçu rapide pour FK506 Binding Protein 2, 13kDa (FKBP2) (AA 22-142) Peptide (ABIN935096)
Antigène
FKBP2
(FK506 Binding Protein 2, 13kDa (FKBP2))
Origine
Humain
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Type de proteíne
- Recombinant
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Activité biologique
- Active
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Protein Region
- AA 22-142
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Séquence
- MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL
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Attributs du produit
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Purified recombinant Human FKBP2 protein
Expression System: E.coli
Bioactivity: Specific activity is > 150 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH8.0 using chymotrypsin. -
Pureté
- > 90 % pure
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Indications d'application
- FKBP2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Procédure de l'essai
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- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
2. Add recombinant FKBP2 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
5. Record the increase in A410 nm for 20 minutes
- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris buffer, pH 8.0, containing 10 % glycerol and 0 mM DTT.
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Agent conservateur
- Dithiothreitol (DTT)
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Précaution d'utilisation
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles.
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Stock
- RT/-20 °C
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Stockage commentaire
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- FKBP2 (FK506 Binding Protein 2, 13kDa (FKBP2))
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Sujet
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FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Recombinant FKBP2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: FKBP 13 protein, FK506 binding protein 2 13kDa protein, FKBP 2, FKBP-2, FKBP13 protein, EC 5.2.1.8 protein, FKBP-13 protein, PPIase protein, Peptidyl prolyl cis trans isomerase protein, FKBP-2 protein, Rotamase., Peptidyl-prolyl cis-trans isomerase FKBP2 13 kDa FKBP protein, FK506 binding protein 2 (13kD) protein, Rapamycin binding protein protein, Proline isomerase protein, FKBP2, FKBP 2 protein, FKBP 2 protein -
Poids moléculaire
- 13.4 kDa (122 AA)
Antigène
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