A synthetic peptide for use as a blocking control in assays to test for specificity of NKIRAS1 antibody, Alternative Names: NKIRAS1 control peptide, NKIRAS1 antibody Blocking Peptide, Anti-NKIRAS1 Blocking Peptide, Nfkb Inhibitor Interacting Ras-Like 1 Blocking Peptide, KBRAS1 Blocking Peptide, kappaB-Ras1 Blocking Peptide, NKIRAS1, NKIRAS-1, NKIRAS 1, NKIRAS-1 Blocking Peptide, NKIRAS 1 Blocking Peptide
Purification
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
NKIRAS1 is an atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. NKIRAS1 may act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.