PrxQ anticorps
L’anticorps Lapin Polyclonal anti-PrxQ a été validé pour WB. Il convient pour détecter PrxQ dans des échantillons de Arabidopsis thaliana, Manihot esculenta, Spinach et Zea mays. Il y a 3+ publications disponibles.
(3 références)
N° du produit ABIN125852
Aperçu rapide pour PrxQ anticorps (ABIN125852)
Antigène
PrxQ (PRXQ)
(Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))
Reactivité
Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays
Hôte
Lapin
Clonalité
Polyclonal
Application
Western Blotting (WB)
-
-
Réactivité croisée (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
-
Homologie
- Populus spp. , monocots including Triticum aestivum, Oryza sativa
-
Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 16 kDA
-
Purification
- serum
-
Immunogène
- His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
-
-
-
Indications d'application
- 1: 5000 with standard ECL (WB)
-
Commentaires
-
In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- For reconstitution add 200 μL of sterile water.
-
Conseil sur la manipulation
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Stock
- -20 °C
-
Stockage commentaire
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
-
-
: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." dans: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).
: "Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts." dans: Journal of experimental botany, (2010) (PubMed).
: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." dans: Journal of plant physiology, (2010) (PubMed).
-
: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." dans: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).
-
- PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))
-
Autre désignation
- PrxQ
-
Sujet
-
AGI Code: At3g26060
Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence. -
Poids moléculaire
- 16 kDA
-
UniProt
- Q9LU86
Antigène
-
Vous êtes ici:
