IFN alpha 2b Protein (Arg46)

N° du produit ABIN1096852

Protéine Recombinant IFN alpha 2b exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IFN alpha 2b Protein (Arg46) (ABIN1096852)

Antigène: IFN alpha 2b

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: Arg46

Fonction: Recombinant Human Interferon α2B Variant (Arg46)/IFN-α2B

Séquence: MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Attributs du produit: Recombinant Human Interferon-alpha2b produced in E. coli is a single non-glycosylated polypeptide chain containing 166 amino acids with a molecular mass of 19,4 kDa. The Interferon-alpha2b gene was obtained from human leukocytes.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Biological Activity Comment: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 3 months

Détail du antigène

Antigène: IFN alpha 2b

Autre désignation: IFN-alpha 2B

Sujet: At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.
Alternative Names: Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha-A, LeIF A, IFNA2

Poids moléculaire: 19.4 kDa

UniProt: P01563