MMP8 Protein (Catalytic Domain)

N° du produit ABIN1097894

Cette protéine Recombinant MMP8 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MMP8 Protein (Catalytic Domain) (ABIN1097894)

Antigène: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Pureté: > 95% by SDS-PAGE.

Détail du produit

Attributs du protein: Catalytic Domain

Séquence: NPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI YG

Attributs du produit: Recombinant matrix metalloproteinase-8 (MMP-8, collagenase-2, neurophil collagenase) cloned from human cDNA, expressed in E.coli. The enzyme consists of the catalytic domain of human MMP-8 (residues 105-262)

Purification: Folding state checked by NMR.

Biological Activity Comment: > 80U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Information d'application

Indications d'application: Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors

Commentaires:

The enzyme was observed as a single band migrating at a molecular weight of < 20kDa

Restrictions: For Research Use only

Stockage

Buffer: 0.2mg/ml in Tris 20mM pH 7.2, CaCl2 10mM, ZnCl2 0.1mM, NaCl 0.3M, Acetohydroxamic Acid (AHA) 0.5M.

Stock: -20 °C/-80 °C

Stockage commentaire: -80°C. The enzyme is stable at -20°C for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80°C. Avoid repeated freeze/defrost cycles.

Détail du antigène

Antigène: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Autre désignation: MMP-8

Poids moléculaire: 17.7kDa

UniProt: P22894