MDH (AA 1-312) (Active) protein (His tag)

N° du produit ABIN1098249

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MDH (AA 1-312) (Active) protein (His tag) (ABIN1098249)

Antigène: MDH

Type de proteíne: Recombinant

Activité biologique: Active

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-312

Purification/Conjugué: His tag

Séquence: MKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Purification: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 1000unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Synonyms: malate dehydrogenase, ECK3225, JW3205

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 50 mM NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: MDH

Sujet: Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E. coli mdh protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 34.9 kDa (336aa) confirmed by MALDI-TOF

NCBI Accession: NP_417703