PykF protein (His tag)

N° du produit ABIN1098253

Protéine Recombinant exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour PykF protein (His tag) (ABIN1098253)

Antigène: PykF

Type de proteíne: Recombinant

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 % by SDS - PAGE

Détail du produit

Purification/Conjugué: His tag

Purification: purified by chromatography

Information d'application

Commentaires:

Synonyms: pyruvate kinase I, ECK1672, JW1666

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PykF

Sujet: PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 53.3kDa (494aa)

NCBI Accession: NP_416191