Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-515) (His tag)

N° du produit ABIN1098613

Protéine Recombinant Glucose-6-Phosphate Dehydrogenase exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-515) (His tag) (ABIN1098613)

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-515

Purification/Conjugué: Cette Glucose-6-Phosphate Dehydrogenase protéine est marqué à la His tag.

Séquence: MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

Purification: > 95% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 45unit/mg, obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Synonyms: glucose-6-phosphate 1-dehydrogenase, G6PD1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Autre désignation: G6PD

Sujet: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in insect cell using baculovirus expression system and purified by using conventional chromatography.

Poids moléculaire: 61.4 kDa (535aa)

NCBI Accession: NP_001035810

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones