MYCN Protein (AA 1-464) (GST tag)

N° du produit ABIN1311728

Détail du produit

Antigène: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Type de proteíne: Recombinant

Attributs du protein: AA 1-464

Origine: Humain

Source: Wheat germ

Purification/Conjugué: Cette MYCN protéine est marqué à la GST tag.

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Fonction: MYCN (Human) Recombinant Protein (P01)

Séquence: MPSCSTSTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW KKFELLPTPP LSPSRGFAEH SSEPPSWVTE MLLENELWGS PAEEDAFGLG GLGGLTPNPV ILQDCMWSGF SAREKLERAV SEKLQHGRGP PTAGSTAQSP GAGAASPAGR GHGGAAGAGR AGAALPAELA HPAAECVDPA VVFPFPVNKR EPAPVPAAPA SAPAAGPAVA SGAGIAAPAG APGVAPPRPG GRQTSGGDHK ALSTSGEDTL SDSDDEDDEE EDEEEEIDVV TVEKRRSSSN TKAVTTFTIT VRPKNAALGP GRAQSSELIL KRCLPIHQQH NYAAPSPYVE SEDAPPQKKI KSEASPRPLK SVIPPKAKSL SPRNSDSEDS ERRRNHNILE RQRRNDLRSS FLTLRDHVPE LVKNEKAAKV VILKKATEYV HSLQAEEHQL LLEKEKLQAR QQQLLKKIEH ARTC

Attributs du produit: Human MYCN full-length ORF ( NP_005369.2, 1 a.a. - 464 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Stockage

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -80 °C

Stockage commentaire: Best use within three months from the date of receipt of this protein.

Référence

Cheng, He, Chen, Cai, Gu, Pan, Duan, Wu, Wu: "P300 Interacted With N-Myc and Regulated Its Protein Stability via Altering Its Post-Translational Modifications in Neuroblastoma." dans: Molecular & cellular proteomics : MCP, Vol. 22, Issue 3, pp. 100504, (2023) (PubMed).

Smith, Moreno, Heaton, Chesler, Pearson, Garrett: "Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma." dans: Molecular oncology, (2015) (PubMed).

Suenaga, Islam, Alagu, Kaneko, Kato, Tanaka, Kawana, Hossain, Matsumoto, Yamamoto, Shoji, Itami, Shibata, Nakamura, Ohira, Haraguchi, Takatori, Nakagawara: "NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3? resulting in the stabilization of MYCN in human neuroblastomas." dans: PLoS genetics, Vol. 10, Issue 1, pp. e1003996, (2014) (PubMed).

Smith, Womack: "A matrix approach to guide IHC-based tissue biomarker development in oncology drug discovery." dans: The Journal of pathology, Vol. 232, Issue 2, pp. 190-8, (2013) (PubMed).

Détail du antigène

Antigène: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Autre désignation: MYCN (MYCN Produits)

Synonymes: MODED Protein, MYCNOT Protein, N-myc Protein, NMYC Protein, ODED Protein, bHLHe37 Protein, Nmyc Protein, Nmyc-1 Protein, Nmyc1 Protein, c-nmyc Protein, Nmuc1 Protein, fb57a02 Protein, nmyc1 Protein, wu:fb57a02 Protein, zgc:85706 Protein, XN-myc Protein, XNmyc Protein, mycn Protein, mycn-a Protein, mycn-b Protein, mycna Protein, xN-myc1 Protein, MYCN proto-oncogene, bHLH transcription factor Protein, v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog Protein, v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived Protein, olfactory receptor 8S1 Protein, MYCN proto-oncogene, bHLH transcription factor S homeolog Protein, MYCN Protein, Mycn Protein, LOC515887 Protein, mycn Protein, mycn.S Protein

Sujet: Full Gene Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Synonyms: MODED,N-myc,NMYC,ODED,bHLHe37

ID gène: 4613

NCBI Accession: NM_005378