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Protein G Protein (HRP)

Cette protéine Protein G est exprimée dans .
N° du produit ABIN1536528

Aperçu rapide pour Protein G Protein (HRP) (ABIN1536528)

Antigène

Voir toutes Protein G Protéines
Protein G

Origine

Streptococcus

Hôte

Veuillez nous consulter SVP

Application

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB), Immunoelectron Microscopy (IEM)
  • Purification/Conjugué

    Cette Protein G protéine est marqué à la HRP.
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  • Indications d'application

    Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including primary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Dot blot: 1:2,000-1:10,000 Western blot: 1:2,000-1:10,000 ELISA: 1:10,000-1:50,000 Immunohistology: 1:1,000-1:10,000

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute the lyophilized power with 1.0 ml of deionized water to a final HRP-Protein G concentration of 1 mg/ml.

    Buffer

    PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal

    Agent conservateur

    Thimerosal (Merthiolate)

    Précaution d'utilisation

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    HRP-Protein G should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freezing and thawing cycles.

    Date de péremption

    24 months
  • Antigène

    Protein G

    Sujet

    Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry.
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