PDGF-AA Homodimer (Homodimer) (Active) Protéine

N° du produit ABIN1589624

Cette protéine Recombinant est produite dans Escherichia coli (E. coli).

Aperçu rapide pour PDGF-AA Homodimer (Homodimer) (Active) Protéine (ABIN1589624)

Antigène: PDGF-AA Homodimer

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE

Détail du produit

Attributs du protein: Homodimer

Fonction: PDGF-AA

Séquence: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Specificité: Chromosomal location:7p22

Attributs du produit: Length (aa):125

niveau d'endotoxine: < 0.1 ng per ug of PDGF-AA

Information d'application

Indications d'application: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Stock: RT,-20 °C,-80 °C

Stockage commentaire: The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: PDGF-AA Homodimer

Autre désignation: PDGF-AA

Sujet: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A

Poids moléculaire: 28.5 kDa

ID gène: 5154

NCBI Accession: NM_002607, NP_002598

UniProt: P04085