PDGF-AB Heterodimer (Homodimer) (Active) Protéine

N° du produit ABIN1589627

Cette protéine Recombinant PDGF-AB Heterodimer est produite dans Escherichia coli (E. coli).

Aperçu rapide pour PDGF-AB Heterodimer (Homodimer) (Active) Protéine (ABIN1589627)

Antigène: PDGF-AB Heterodimer

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE

Détail du produit

Attributs du protein: Homodimer

Fonction: Recombinant Human PDGF-AB Protein

Séquence: Alpha chain: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Specificité: Chromosomal location:7p22/22q13.1

Attributs du produit: Length (aa):126/110

niveau d'endotoxine: < 0.1 ng per ug of PDGF-AB

Information d'application

Indications d'application: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 mM acetic acid,Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening

Stock: -20 °C,-80 °C

Stockage commentaire: The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AB is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 2-3 weeks

Détail du antigène

Antigène: PDGF-AB Heterodimer

Autre désignation: PDGF-AB

Sujet:

Synonyms: PDGFA, PDGF1, PDGF-A

Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).

Poids moléculaire: 25.5 kDa

ID gène: 5154, 5155

NCBI Accession: NM_002608, NM_002607, NP_002599, NP_002598

UniProt: P04085