AHSA1 Protéine

N° du produit ABIN1686715

Cette protéine Recombinant AHSA1 est exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour AHSA1 Protéine (ABIN1686715)

Antigène: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Functional Studies (Func)

Pureté: >90%

Détail du produit

Séquence: SHMAKWGEGD PRWIVEERAD ATNVNNWHWT ERDASNWSTD KLKTLFLAVQ VQNEEGKCEV TEVSKLDGEA SINNRKGKLI FFYEWSVKLN WTGTSKSGVQ YKGHVEIPNL SDENSVDEVE ISVSLAKDEP DTNLVALMKE EGVKLLREAM GIYISTLKTE FTQGMILPTM NGESVDPVGQ PALKTEERKA KPAPSKTQAR PVGVKIPTCK ITLKETFLTS PEELYRVFTT QELVQAFTHA PATLEADRGG KFHMVDGNVS GEFTDLVPEK HIVMKWRFKS WPEGHFATIT LTFIDKNGET ELCMEGRGIP APEEERTRQG WQRYYFEGIK QTFGYGARLF

Specificité: ~38 kDa

Attributs du produit: 2 µM ABIN1686714 generated a 9-fold ATPase activation of 2 µM Hsp90 (His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Purification: Multi-Step Purified

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product has been certified >90% pure using SDS PAGE analysis. 2uM ABIN1686714 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Restrictions: For Research Use only

Stockage

Concentration: Lot specific

Buffer: 20 mM HEPES buffer pH 7.2, 80 mM NaCl, 10 % glycerol

Stock: -20 °C

Référence

Margarucci, Monti, Cassiano, Mozzicafreddo, Angeletti, Riccio, Tosco, Casapullo: "Chemical proteomics-driven discovery of oleocanthal as an Hsp90 inhibitor." dans: Chemical communications (Cambridge, England), Vol. 49, Issue 52, pp. 5844-6, (2013) (PubMed).

Détail du antigène

Antigène: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Autre désignation: Aha1

Sujet: Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Poids moléculaire: approx. 38 kDa

ID gène: 10598

NCBI Accession: NP_036243

UniProt: O95433