LacI (AA 1-360) protein (His tag)

N° du produit ABIN1692437

Cette protéine Recombinant LacI est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour LacI (AA 1-360) protein (His tag) (ABIN1692437)

Antigène: LacI

Type de proteíne: Recombinant

Origine: E. coli

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-360

Purification/Conjugué: His tag

Fonction: Recombinant E.coli LacI (C-6His)

Séquence: MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH

Attributs du produit: Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as a 0.2 um filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 5 mM DTT pH 8.0 .

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: minimize freeze-thaw cycles

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: LacI

Sub Type: Fusionprotein

Sujet: Alternative Names: Lactose operon repressor, LacILactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Alternative Names: Lactose operon repressor, LacI

Poids moléculaire: 39.4 kDa

UniProt: P03023