Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in sterile PBS, pH 7.2 - pH 7.4.
Buffer
Supplied as lyophilized form in 50 mM TRIS, 200 mM NaCl
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles
Stock
4 °C
Stockage commentaire
Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
H4/p Protein, histone cluster 4 H4 Protein, HIST4H4 Protein
Sujet
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element.