Host-Nuclease Inhibitor Protein Gam (AA 1-138) Protéine
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Antigène
- Host-Nuclease Inhibitor Protein Gam
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-138
- Origine
- Souris
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Source
- Escherichia coli (E. coli)
- Fonction
- Recombinant Mouse Sonic Hedgehog/SHH (C25II)
- Séquence
- MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
- Attributs du produit
- Recombinant Bacteriophage Host-nuclease inhibitor protein gam is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val138) of Bacteriophage GAM fused with a 6His tag at the C-terminus.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- Stérilité
- 0.2 μm filtered
- niveau d'endotoxine
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.
- Agent conservateur
- Dithiothreitol (DTT)
- Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Stock
- 4 °C/-20 °C/-80 °C
- Stockage commentaire
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- Host-Nuclease Inhibitor Protein Gam
- Sujet
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Host-nuclease inhibitor protein gam(gam) comes from Enterobacteria phage lambda. Originally the gam gene product was assigned to the sequence shown and it was suggested that the gene kil protein is encoded from within this gene and corresponds to residues 41-138. For an alternative assignment of this gene see the entry for the kil gene protein of lambda. The function of the protein inhibits the host recBC-coded nuclease and thus promotes the late, rolling-circle mode of lambda DNA replication.
Synonyms: Host-nuclease inhibitor protein gam,gamma - Poids moléculaire
- 19.8 kDa
- UniProt
- Q62226
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