Cathepsin G Protein (CTSG)

N° du produit ABIN2128355

Cette protéine Native Cathepsin G est produite dans Human. Elle a été validée indépendamment.

Aperçu rapide pour Cathepsin G Protein (CTSG) (ABIN2128355)

Antigène: Cathepsin G (CTSG)

Type de proteíne: Native

Origine: Humain

Source: Human

Pureté: > 96 % pure

Détail du produit

Information d'application

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102720 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102720

Date: 05.11.2018

Antigène: CTSG

Numéro du lot: A14041801

Application validée: Unfolding Profile

Contrôle positif: ABIN2128355

Conclusion:

Passed. ABIN2128355 showed a T_i of 63.8 °C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Validation Images

Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 63.8°C.

Protocole

Full Protocol:

• Dilute ABIN2128355 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.5µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Liquid 50 mM sodium acetate, pH 5.5, 0.8 M sodium chloride

Stock: 4 °C/-20 °C/-80 °C

Détail du antigène

Antigène: Cathepsin G (CTSG)

Autre désignation: Cathepsin G

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones