VEGF Protein (AA 27-191) (Biotin)

N° du produit ABIN2181906

Protéine Recombinant VEGF exprimée dans HEK-293 Cells et a été mentionnée dans 1 publication.

Aperçu rapide pour VEGF Protein (AA 27-191) (Biotin) (ABIN2181906)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: >95 % as determined by reduced SDS-PAGE.

Détail du produit

Attributs du protein: AA 27-191

Purification/Conjugué: Cette VEGF protéine est marqué à la Biotin.

Marque: MABSol®,UltraLys

Séquence: AA 27-191

Specificité: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Attributs du produit: The product does NOT contain any epitope tags. The protein has a calculated MW of 19 kDa (monomer). The protein migrates as 24 kDa (monomer) on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 43-50 kDa under non-reducing (NR) condition.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Référence

McGonigle, Majumder, Kolber-Simonds, Wu, Hart, Noland, TenDyke, Custar, Li, Du, Postema, Lai, Twine, Woodall-Jappe, Nomoto: "Neuropilin-1 drives tumor-specific uptake of chlorotoxin." dans: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 67, (2019) (PubMed).

Détail du antigène

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Autre désignation: VEGF

Sujet: VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.

Poids moléculaire: 19.2 kDa

NCBI Accession: NP_001165097