Botulinum C3 Enzyme (Active) Protéine
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- Antigène
- Botulinum C3 Enzyme
- Type de proteíne
- Native
- Activité biologique
- Active
- Origine
- Clostridium botulinum
- Source
- Clostridium botulinum
- Application
- Functional Studies (Func)
- Pureté
- > 90 % purity by SDS-PAGE (CBB staining)
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- Indications d'application
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1) For the studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro.
2) ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
Activity: Stimulation of human keratinocytes in culture was observed at 50 ng/mL. - Commentaires
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No toxic effect was observed in two mice injected with 5 ug of C3 enzyme, showing little contamination of lethal neurotoxins from the botulinus culuture in this preparation.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.33 mg/mL
- Buffer
- 5 mM sodium phosphate buffer ( pH 6.0), 50 % glycerol
- Stock
- -20 °C/-80 °C
- Stockage commentaire
- -20 C (For long term storage: -70 C)
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Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation." dans: Cell death and differentiation, Vol. 5, Issue 9, pp. 720-8, (1999) (PubMed).
: "Purification and characterization of ADP-ribosyltransferases (exoenzyme C3) of Clostridium botulinum type C and D strains." dans: Journal of bacteriology, Vol. 173, Issue 19, pp. 6025-9, (1991) (PubMed).
: "
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Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation." dans: Cell death and differentiation, Vol. 5, Issue 9, pp. 720-8, (1999) (PubMed).
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- Antigène
- Botulinum C3 Enzyme
- Sujet
- This product was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns. This toxin ADP-ribosylates Rho and Rac, which are low-molecular weight GTP-binding proteins of animal cells, and interrupts the downstream signal transduction pathways. The molecular weight is 24 kDa.
- UniProt
- P15879
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