RNASEH Protéine

N° du produit ABIN3200996

Détail du produit

Antigène: RNASEH (Ribonuclease H (RNASEH))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: E. coli

Source: Escherichia coli (E. coli)

Attributs du produit: Specific Activity: 100, 000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37 C.

Pureté: > 95 % protein determined by SDS-PAGE (CBB staining)

Information d'application

Indications d'application: 1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 50 units/L

Buffer: 50 units/ul in 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 1 mM DTT, 50 % Glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Référence

Gubler: "Second-strand cDNA synthesis: mRNA fragments as primers." dans: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).

Donis-Keller: "Site specific enzymatic cleavage of RNA." dans: Nucleic acids research, Vol. 7, Issue 1, pp. 179-92, (1979) (PubMed).

Vournakis, Efstratiadis, Kafatos: "Electrophoretic patterns of deadenylylated chorion and globin mRNAs." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 8, pp. 2959-63, (1976) (PubMed).

Détail du antigène

Antigène: RNASEH (Ribonuclease H (RNASEH))

Abstract: RNASEH Produits

Synonymes: BA1623 Protein, rnhA Protein, SC5H1.08c Protein, ribonuclease H Protein, ribonuclease HI Protein, Ribonuclease H Protein, rnhA Protein, SCO7284 Protein, CBU_0316 Protein, CNA03470 Protein, CNN02200 Protein, Tb10.70.2140 Protein, Tmath_1295 Protein, Deima_2376 Protein, Tresu_0232 Protein, Marky_0915 Protein, Desac_1125 Protein, Corgl_1288 Protein

Sujet: Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.

UniProt: P0A7Y4