RecA (Active) Protéine

N° du produit ABIN2452179

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 2+ publications.

Aperçu rapide pour RecA (Active) Protéine (ABIN2452179)

Antigène: RecA

Type de proteíne: Recombinant

Activité biologique: Active

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Pureté: > 90 % by SDS-PAGE (CBB staining)

Détail du produit

Attributs du produit: The product is over-expressed as a recombinant protein and highly purified by several steps of chromatography. A single band is observed by SDS-PAGE at 38 kD.

Information d'application

Indications d'application: 1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (2).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.6 mg/mL

Buffer: 20 mM Tris-HCl ( pH 8.0), 1 mM EDTA, 150 mM KCl, 1 mM DTT, 50 % glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20 C (For long term storage: -70 C)

Référence

Walker: "Understanding the complexity of an organism's responses to DNA damage." dans: Cold Spring Harbor symposia on quantitative biology, Vol. 65, pp. 1-10, (2003) (PubMed).

Taidi-Laskowski, Tyan, Honigberg, Radding, Grumet: "Use of RecA protein to enrich for homologous genes in a genomic library." dans: Nucleic acids research, Vol. 16, Issue 16, pp. 8157-69, (1988) (PubMed).

Détail du antigène

Antigène: RecA

Sujet: E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein.

UniProt: P0A7G6