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RuvC Protéine

RuvC Origine: E. coli Hôte: Escherichia coli (E. coli) Native SDS, Func, ELISA, WB Active
N° du produit ABIN2452186
  • Antigène Tous les produits RuvC
    RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
    Type de proteíne
    Native
    Activité biologique
    Active
    Origine
    E. coli
    Source
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
    Attributs du produit
    Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
  • Indications d'application
    1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
    2) SDS-PAGE (0. 2 g/lane)
    3) Standard antigen for western blotting and ELISA
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
    Stock
    -20 °C/-80 °C
    Stockage commentaire
    Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
  • Murayama, Kurokawa, Mayanagi, Iwasaki: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." dans: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).

    Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." dans: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).

    Iwasaki, Takahagi, Shiba, Nakata, Shinagawa: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." dans: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).

  • Antigène
    RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
    Autre désignation
    RuvC (RuvC Produits)
    Synonymes
    ECK1864 Protein, JW1852 Protein, Crossover junction endodeoxyribonuclease RuvC Protein, ruvC Protein
    Sujet
    E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
    UniProt
    P0A814
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