T4 SSB (Active) Protéine

N° du produit ABIN2452207

Protéine Recombinant exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 2+ publications.

Aperçu rapide pour T4 SSB (Active) Protéine (ABIN2452207)

Antigène: T4 SSB

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Bacteriophage T4

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Pureté: > 95 % of protein determined by SDS-PAGE (CBB staining)

Détail du produit

Attributs du produit: The absence of endonucleases and exonucleases was confirmed.

Information d'application

Indications d'application: 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Référence

Bittner, Burke, Alberts: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." dans: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).

Alberts, Frey: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." dans: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).

Détail du antigène

Antigène: T4 SSB

Classe de substances: Phage Protein

Sujet: T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.

UniProt: P03695