T4 SSB (Active) Protéine
Origine: Bacteriophage T4
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % of protein determined by SDS-PAGE (CBB staining)
Func
Active
(2 références)-
- Antigène
- T4 SSB
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Origine
- Bacteriophage T4
- Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func)
- Attributs du produit
- The absence of endonucleases and exonucleases was confirmed.
- Pureté
- > 95 % of protein determined by SDS-PAGE (CBB staining)
-
- Indications d'application
-
1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 10 mg/mL
- Buffer
- 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
- Agent conservateur
- Dithiothreitol (DTT)
- Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
-
-
: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." dans: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).
: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." dans: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).
-
: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." dans: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).
-
- Antigène
- T4 SSB
- Classe de substances
- Phage Protein
- Sujet
- T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
- UniProt
- P03695
-
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